Let's Grow Mushrooms Together (part 2)
Hello growing enthusiasts! I welcome you back to another post of growing mushrooms together. There may have been something wrong with the spore syringe I inoculated four of the eight jars with. To be honest it was hard to see any spores in the syringe before shaking it up and I really didn't notice many in it afterwards either but I figured they were ok because it was from the same company I used for the last grow and I did a decent job on the last spores I got from them. But two of my jars didn't have any colonization in them whatsoever so today is a little bonus for you guys of how I made a spore syringe from a spore print. This will be good for you guys that only have prints. This is how I did it.....
First I had to deal with the other four jars that had a massive and really fast colonization due to being a culture syringe oppose to just a spore syringe. All I did was wait until they are at least a third colonized and hit them against the palm of my hand until the mycelium was broken up and mixed thoroughly throughout the jars 👇
This is what it looks like after mixing the mycelium ☝️ You can see how good it mixed and that is to help the mycelium colonize faster and evenly in the jars. Those four went back into the cupboard.
Next I noticed only two of the four other jars had any mycelium started in them so I will explain what I did to reinoculate those two jars from one of my spore prints I took in one of my previous posts.
First I gathered all my syringes I had from past grows and washed them with soapy antibacterial soap and put them in a big mason jar 👇
and took one of my pint jars and filled it half way with water from my zero water pitcher. 👇
you can use any kind of water but if I don't have any distilled water I'll use water from my Zero Water Pitcher because it's the only filtration pitcher I've found that takes all dissolved solids from the water. This picture shows the Total Dissolved Solids (TDS) in the water from the pitcher 👇
After putting the lids and bands on the two jars I unscrew the band a quarter turn to allow for the sterilization process to enter my jars. I cover the lids with aluminum foil and put them in my pressure canner. I turned the flame on high until the recommended pressure (15psi) happens and then turned down the flame to medium heat to keep the pressure at 15psi and started a timer for 30 minutes. 👇
The reason why I use the pressure canner is because boiling alone cannot get the temperature high enough to sterilize the jars. Boiling point of water is only 212 degrees Fahrenheit at sea level. To get it to sterilization temps it must get to at least 250 degrees and held for a certain amount of time and the only way to get that is under pressurized conditions.
Once the time is completed I leave the PC to depressurize on it's own which usually lasts for about an hour or so and let the jars cool down to room temperature before I take them out of the PC which is usually the next morning.
Next I'll get my still air box and clean it inside and out with 70% isopropyl alcohol. I grabbed a sterile syringe, a paper towel sprayed with alcohol, a knife to scrape the spores with, my jar of water, the jars to be inoculated, my spore print and a flame to sterilize the needle in between each inoculation.
Notice the second and fourth jar with nothing happening ☝️
When working in mycology you should do everything possible to prevent contaminants even when taking a print. Everything should be done in front of a flow hood or in a still air box. I don't have the means right now to build a flow hood so all I have is my still air box which is ok to use. Don't get discouraged if you get contaminants doing mycology because even the most sterile mycologists gets contams once in a while. Use it as a learning experience and try to minimize the amount of contaminants that is presented by taking a shower beforehand, using gloves, covering face and exposed skin, and using alcohol for everything! Alcohol is your best friend when doing mycology! Ok on with the show 😉
When I have all my things in my SAB 👇
I spray my gloves and wipe down all my instruments with alcohol. I open up the print and scrape the print into the jar of water with the knife and quickly close with the lid. 👇
After tightening the lid I shake the jar really good to break up any spores that may be stuck together and prepare the syringe. There are billions of spores on one print which are microscopic so don't think there isn't enough in the jar after doing this! After wiping down the syringe with the alcohol I flame sterilize the needle 👇 ) Be sure to get the needle red hot. Let it cool down a bit and suck up a full syringe from the jar of spore solution. Put the needle cap back on 👇 and give it a shake. )
Grab the grain jars you'll be inoculating. Wipe the jars of grain down with the alcohol wet paper towel. Flame sterilize the needle again and inject each jar with 2 1/2 cc's of solution with a flame sterilize in between each jar. Put the cap back on and keep the spore syringe and the spore solution jar in your fridge for future use. In the fridge they will last for 2-4 years. If you're anything like me they won't last that long!
Well that's part 2 of growing mushrooms together and I hope you come back for part three when we talk about putting the colonized grain spawn to bulk substrate! Until then Mush Love!
How long do you think a syringe is good for outside of fridge?
I've heard of them lasting for years at room temp and still being good but personally I don't know. I've always heard the best way to prolong the spores in sterile solution is in the fridge. Hope this helps
Awesome. Can’t wait to see when these are ready to pick.
You and me both bro!
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A very professional job and a good guide. It does not look easy to do. Now I understand why some friends of mine used to go to the mountain to look for Phislocybes in cow dung, lol.
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Thank you bro! It really isn't hard to do, it takes practice. 😉🍄
Cant wait to give this a try. I am still a bit intimadated, but I really am appreacitated your posts. Once I do it, that is when I really learn.
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That's the only way you can get better at something is to keep trying, and hey if you mess up at least you know a guy that will hook you up with more! 😉😆
That’s music to my ears! Really glad you found and are taking a liking to this place ! :)
I really do love being here man! I love that everyone shares their experiences and we all can learn from one another!
love that this post popped up when I've been meaning to take some prints for a while now...
I like to mooooove it mooooooove it
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Very awesome! Everything happens at the right time!
I have a couple questions about the stuff in the pics. It looks like you took the spore print on foil. Can you address the process you use to take a spore print? Also, what is the device you used to determine the water was free of particulates?
Of course bro! What I did was pick out a nice mushroom that opened up and clipped the stem off and put the cap spore side down on aluminum foil inside my still air box and left it over night. You can see in my previous post here... https://ecency.com/hive-195708/@anewyorkminute79/mushroom-grow-part-2 I forgot to blog this post.
And the meter I'm using actually came with the zero water pitcher but it's called a TDS meter which measures the total dissolved solids in the water. You can use any water but it may change colors due to having dissolved solids in your water when doing culture. It won't harm anything by using tap water. I'm just over cautious with everything lol I hope this helped.
I find this entire process to be very fascinating on so many levels. The colonization process starts pretty quickly if the environment/substrate is perfect. Your guide is really nicely put together and detailed in an easy to understand way. Thank you for taking the time to put this together so others can learn from your work!
Thank you bro! I'm trying to explain as easy as I can so anyone can grow and benefit from the beautiful fungi! You're very welcome man!
Yeah some of the crazy teks are awesome.
The orbies one? Wow.
And these... It's penis envy and b+ cross and mutation.
I can't wait to see more!
Lol I've had so many mushrooms I'm saving for a special day.